Sterol Ferulate Rich Wheat Bran Oil Concentrate Prevents Liver Damage in MAFLD Mouse Model by Modulating FAS and PCSK9

Molecular effects of sterol-enriched wheat bran oil (eWBA) on lipid metabolism, inflammatory markers, and liver proteome associated with nonalcoholic/metabolic-related fatty disease (MAFLD) are not completely known. In this study, dietary eWBA intervention was used to investigate its effect in ameliorating injury high fat diet (HFC)-fed C57BL/6 mice. Specifically, we examined eWBA’s (i) differentially expressed hepatic factors which regulate carbohydrate homeostasis such as sterol regulatory element-binding protein-2 (SREBP-2), peroxisome proliferator-activated receptors-γ (PPARγ), pro-protein convertase subtilisin/kexin type-9 (PCSK9), low-density lipoprotein receptor (LDL-R), apolipoprotein-E (APO-E) factors, (ii) levels two cytokines, tumor necrosis factor (TNF-α) transforming growth beta (TGF-β). Liver HFC-fed mice exhibited characteristic features MAFLD, increased serum markers (SGOT, SGPT), lipids, PCSK9 protein. Mice fed eWBA-supplemented HFC amelioration cholesterol, triglycerides, SGOT, SGPT well a reduction lipids SREBP-2 proteins. Pro-inflammatory pro-fibrogenic cytokines the groups - supplemented were normalized respect group without eWBA. To conclude, reduces expression SREBP-2, APO-E, down regulates various signal molecules thereby ameliorates mouse model MAFLD. Down-regulated FAS ZNF-236 proteins could be initial for steatosis.